DNA

Part:BBa_K4747020:Design

Designed by: Liu Lantian   Group: iGEM23_SUSTech-MED   (2023-10-11)


araC-araBAD-xisF4-PA0717-PA0718-PA0719-PA0720-PA0722-CoaB-PA0724-PA0725-yhjF-PA0727


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5484
    Illegal EcoRI site found at 7209
    Illegal XbaI site found at 6382
    Illegal PstI site found at 7715
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5484
    Illegal EcoRI site found at 7209
    Illegal NheI site found at 7271
    Illegal NheI site found at 8173
    Illegal PstI site found at 7715
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5484
    Illegal EcoRI site found at 7209
    Illegal BglII site found at 310
    Illegal BamHI site found at 5505
    Illegal BamHI site found at 5625
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5484
    Illegal EcoRI site found at 7209
    Illegal XbaI site found at 6382
    Illegal PstI site found at 7715
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5484
    Illegal EcoRI site found at 7209
    Illegal XbaI site found at 6382
    Illegal PstI site found at 7715
    Illegal NgoMIV site found at 974
    Illegal NgoMIV site found at 3270
    Illegal NgoMIV site found at 3396
    Illegal NgoMIV site found at 3406
    Illegal NgoMIV site found at 5744
    Illegal NgoMIV site found at 6051
    Illegal NgoMIV site found at 7314
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 3298
    Illegal SapI.rc site found at 823
    Illegal SapI.rc site found at 1033
    Illegal SapI.rc site found at 5251


Design Notes

To perform genetic engineering of prophage pf4 in Pseudomonas aeruginosa PAO1, inserting yhjH downstream of the PA0726


Source

The yhjH gene, encoding the phosphodiesterase (PDE) of the c-di-GMP pathway, was adeptly integrated within PA0726 aiming to mitigate biofilm formation, under the regulatory ambit of the arabinose operon.

References